"In-Vitro Testsystems - From the tube to the living cell"
Activation and lipid mediator release of basophils after allergen exposure
Primary cells (white blood cells, glomerular mesangial cells) and cancer cell lines (colon, breast) as models for cytokine production, activation of inflammation and apoptosis and for the extracellular matrix and its composition
Burkitt lymphoma lines for optimizing drug delivery and therapy efficiency of virus-based gene delivery
Keratinocytes: Primary human cells and cell lines derived from keratinocyte tumors to assess phospholipase A2 and its role in cancer
Determination of platelet activity and hemostasis after administration of NSAID. Electrophysiological determination of pain-related pathways by Patch Clamp analysis and Calcium Imaging in cell cultures of spinal cord neurons and dorsal root ganglia. Studying of intestinal permeability and absorptive/secretory transport processes with the patch-clamp technique Single cell immune assays to determine dendritic cell maturation parameters and antigen-specific T lymphocyte responses. 96 well luminometer for „High Throughput“ gene Reporter Assay to investigate presence and functionality of transcription inducing or repressing proteins. 96 well Neuroprobe® chamber based chemotaxis assay with semi-automatic evaluation and circular flow chamber to assess effects of anti-inflammatory recombinant and biological therapeutics. Neuronal models for Alzheimer Alzheimer’s disease & oxidative stress Blood-brain barrier models Receptor related neuronal transmission Using the Caco-2 permeability assay for rapid assessment of the absorption potential and susceptibility of drugs for cellular efflux pumps Identification of disease relavant molecules in patiens with rheumatic disease: The step from the animal model to humen tissue Development of test systems for antiinflammatory drug screening In-vitro assay for transcription/translation of new inhibitors for bacterial protein synthesis (antibiotics) Testing cytotoxicity of cytostatic drugs in combination with immune effector cells, cellular proliferation and function by five colour-flow cytometry. Biorelevant dissolution testing Functional analysis of MLL fusion proteins in eukaryotic cells Testing oncogenic potential of human proteins in eukaryotic model systems Jumping genes and their control: site-specific integration into genomes Testing human embryo toxicity in eukaryotic model systems Identifying new ligands in radio-ligand binding assays Identifying Ras-Raf inhibitors by Yeast-2-Hybrid screens Use of transient and stable transfection studies to analyze gain-of functions in mammalian cell systems PLK inhibition by siRNA in combination with cytostatic drug treatment of breast cancer cell lines Determination of the effect of de-methylating agents in combination with cytostatic drug treatment on breast cancer cell lines
