Institute for pharmaceutical
chemistry -
Prof. Dr. D. Steinhilber |
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Profile
The group of Prof. Dr. Steinhilber
focuses on the elucidation of
mechanisms which are involved
in inflammatory diseases and
pain. The studies are done on
DNA and Protein level, both
under structural aspects (identification
of DNA sequences/ proteins)
and functional points of view
(regulation of DNA transcription/
protein activity).
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Expertise
- Inflammation research (special
focus on arachidonic acid
cascade)
- Signal transduction
- Investigation of regulation
of gene expression
- Studies of nuclear receptors
and epigenetic mechanisms
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Resources
and Abilities
- 2D-Gel elektrophoresis
- Chromatography/ protein
purification
- Cloning of cDNA and protein
expression in eucaryontic
and procaryontic sytems
- Immunohistochemistry
- HPLC analytics of eicosanoids
- Cellular assays (reactive
oxygen species, kinase assays,
calcium imaging, different
functional assays)
- Cell culture techniques
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Publications
- Straif, D., Werz, O., Kellner,
R., Bahr, U. and Steinhilber,
D. (2000) Glutathione peroxidase-1
but not 4 is involved in the
regulation of cellular 5-lipoxygenase
activity in monocytic cells.
Biochem J 349, 455-461
- Steinmetz, R.D., Fava, E.,
Nicotera, P. and Steinhilber,
D. (2002) A simple cell line
based in vitro test system
for N-methyl-D-aspartate (NMDA)
receptor ligands. J Neurosci
Methods 113, 99-110
- Werz, O., Wiesinger, H.,
Steinmeyer, A. and Steinhilber,
D. (2000) New vitamin D receptor
agonists with decreased metabolic
stability. Biochem Pharmacol
59, 1597-1601
- Härle, D., Rädmark,
O., Samuelsson, B. and Steinhilber,
D. (1998) Calcitriol and transforming
growth factor-beta upregulate
5-lipoxygenase mRNA expression
by increasing gene transcription
and mRNA maturation. Eur J
Biochem 254, 275-281
- Werz, O., Bürkert,
E., Rådmark, O. and
Steinhilber, D. (2002) Activation
of 5-lipoxygenase by cell
stress is calcium independent
in human polymorphonuclear
leukocytes. Blood 99, 1044-1052
- Uhl, J., Klan, N., Rose,
M., Entian, K.D., Werz, O.
and Steinhilber, D. (2002)
The 5-lipoxygenase promoter
is regulated by DNA methylation.
J Biol Chem 277, 4374-4379
- Werz, O., Szellas, D., Steinhilber,
D. and Rådmark, O. (2002)
Arachidonic acid promotes
phosphorylation of 5-lipoxygenase
at Ser-271 by MAPK-activated
protein kinase 2 (MK2). J
Biol Chem 277, 14793-14800
- Werz, O., Bürkert,
E., Fischer, L., Szellas,
D., Dishart, D., Samuelsson,
B., Rådmark, O. and
Steinhilber, D. (2002) Extracellular
signal-regulated kinases phosphorylate
5-lipoxygenase and stimulate
5-lipoxygenase product formation
in leukocytes. FASEB J 16,
1441-1443
- Klan, N. and Steinhilber,
D. (2003) Transient transfection
of the human myeloid cell
line Mono Mac 6 using electroporation.
Biotechniques 34, 142-147
- Klan, N., Seuter, S., Schnur,
N., Jung, M. and Steinhilber,
D. (2003) Trichostatin A and
structurally related histone
deacetylase inhibitors induce
5-lipoxygenase promoter activity.
Biol Chem 384, 777-785
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Staff:
- PD Dr. rer. nat . Oliver
Werz (protein biochemistry/
signal transduction, new mechanisms
of drug action)
- Dr. rer. nat. Bernd Sorg
(molecular biology/ Vitamin
D-signal transduction)
- Dr. rer. nat. Beate Firla
(molecular biology/ nuclear
receptors)
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ZAFES-Coordinator:
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