Institute of pharmaceutical biology -
Prof. Dr. Theo Dingermann / Prof. Dr. Rolf Marschalek

 

>

Expertise

  • Genomic instability mediated through transposable and retrotransposable elements
  • Retrotransposons and other mobile genetic elements
  • New strategies to improve vector integration specificity in gene therapy
  • The use of Dictyostelium as expression system for recombinant proteins
  • The test of embryonic toxicity in Dictyostelium
  • Target identification for known and novel active substances
  • Acute leukaemia in humans (hematooncology)
  • Pathomolecular mechanisms in the formation of leukaemia
  • Genetic instabilities
  • Mechanisms in gene regulation – expression profiling
  • Diagnostic of human diseases

>

Resources and Abilities

  • Production of monoclonal antibodies
  • 2D-gel electrophoresis
  • mass spectrometry
  • functional test systems
  • developmental toxicology
  • functional test systems
  • exploration of novel targets
    - identification of novel tissue specific target proteins for known and novel active   substances (effects, metabolism, elimination)
  • DNA sequencing, cloning transfection/ transformation
  • Eukaryotic cell cultures (yeast, Dictyostelium, mouse, human)
  • Protein interactions (human, yeast)
  • Protein biochemistry/ protein structure/ molecular mechanims (human)
  • expression of recombinant proteins in bacteria , yeast, Dictyostelium, human cell culture
  • Molecular diagnostic with PCR and FACS

 

>

Publications

  • Szafranski K, Dingermann T, Glöckner G , and Winckler T. Template jumping by a Dictyostelium LINE reverse transcriptase created a SINE-like 5S rRNA retropseudogene, Mol. Genet. Genom. 2004 (accepted)

  • Classen B, Csávás M, Borbás A, Dingermann T, and Zündorf I. Monoclonal antibodies against an arabinogalactan-protein from pressed juice of Echinacea purpurea. Planta Med. 2004 (accepted)

  • Bursen A, Moritz S, Gaussmann A, Dingermann T, Marschalek R. (2003). Deciphering the t(4;11) pathomechanism”. Submitted.

  • Keller JH, Karas M, Müller WE, Volmer DA, Eckert GP, Tawab MA, Blume HH, Dingermann T, and Schubert-Zsilavecz T. Determination of Hyperforin in Mouse Brain by High-Performance Liquid Chromatography/Tandem Mass Spectrometry. Anal. Chem. 2003; 75 (22):6084-608

  • Meyer C, Marschalek R. (2003). Akute Hochrisiko-Leukämie. BioForum 9/2003, 526-528.

  • Schraets D, Lehmann T, Dingermann Th, Marschalek R. (2003). MLL mediated transcriptional gene regulation investigated by gene expression profiling. Oncogene 22, 3655-3668

  • Lühe A, Hildebrand H, Bach U, Dingermann T, Ahr HJ. A New Approach to Studying Ochratoxin A (OTA)-Induced Nephrotoxicity: Expression Profiling in Vivo and in Vitro Employing cDNA Microarrays. Toxicol. Sci. 2003; 73:315-328

  • Böhme TM, Klein C, Kreutzmann K, Linder M, Dingermann T, Dannhardt G, Mutschler E, Lambrecht G. Structure-activity relationships of dimethindene derivatives as new M2-selective muscarinic receptor antagonists. J. Med. Chem. 2003, 46:856-86

  • Dannat K, Tillner J, Winckler T, Weiß M, Eger K, and Dingermann, T. The effects of medicinal compounds on the differentiation of the eukaryotic microorganism Dictyostelium discoideum: Can this model be used as a screening test for reproductive toxicity in humans? Pharmazie 2003; 58:204-210

  • Glöckner G, Eichinger L, Szafranski K, Pachebat J, Dear P, Lehmann R, Baumgart C, Abril JF, Parra G, Guigó R, Kumpf K, Tunggal, Cox E, Quail MA, Platzer M, Rosenthal A, Noegel AA, and the Dictyostelium Genome Sequencing Consortium (u.a. Dingermann T, Winckler T). Sequence and analysis of chromosome 2 of Dictyostelium discoideum, Nature 2002; 418:79–85

  • König M, Reichel M. Marschalek R, Haas OA, Strehl S. A highly specific and sensitive FISH assay for the detection of t(4;11)(q21;q23) in acute leukemias. British Journal of Haematology 2002; 116, 758-764.

  • Friese A, Hell-Momeni K, Zündorf I, Winckler T, Dingermann T, and Dannhardt G. Synthesis and biological evaluation of cycloalkylidene carboxylic acids as novel effectors of Ras/Raf interaction, J. Med. Chem. 2002; 45:1535-1542

  • Beck P, Dingermann T, and Winckler T. Transfer RNA gene-targeted retrotransposition of Dictyostelium TRE5-A into a chromosomal UMP synthase gene trap, J. Mol. Biol. 2002; 318:273-285 (MOLEKULARBIOLOGIE)

  • Hentschel U, Zündorf I, Dingermann T, and Winckler T. On the problem of establishing the subcellular localization of Dictyostelium retrotransposon TRE5-A proteins by biochemical analysis of nuclear extracts, Anal. Biochem. 2001; 296:83-91

  • Fasching K, Panzer S, Haas OA, Borkhardt A, Marschalek R, Griesinger F, Panzer-Grümayer ER. (2001). The Presence of N Regions in the Clonotypic DJ Rearrangements of the Ig Heavy Chain Genes Indicate an Exquisitely Short Latency in t(4;11) Positive Infant Acute Lymphoblastic Leukemia. Blood 2001; 98, 2272-2274

  • Glöckner G, Szafranski K, Winckler T, Dingermann T, Quail M, Cox E, Eichinger L, Noegel AA, and Rosenthal A. The complex repeats of Dictyostelium discoideum, Genome Res. 2001; 11:585-594

  • Winckler T, Trautwein C, Tschepke C, Neuhäuser C, Zündorf I, Vogel G, and Dingermann T. Gene function analysis by stop codon suppression: CMBF is a nuclear protein that supports growth and development of Dictyostelium amoebae, J. Mol. Biol. 2001; 305:703-714

  • Hensel JP, Gillert E, Fey GH, Marschalek R. (2001). Breakpoints of t(4;11) translocations in the human MLL and AF4 genes in ALL patients are preferentially clustered outside of high-affinity matrix attachment regions. Journal of Cellular Biochemistry 2001; 82, 299-309

  • Reichel M, Gillert E, Angermüller S, Hensel JP, Heidel F, Lode M, Leis T, Biondi A, Haas OA, Strehl S, Panzer-Grümayer ER, Griesinger F, Beck JD, Greil J, Fey GH, Uckun FM, Marschalek R. Biased distribution of chromosomal breakpoints involving the MLL gene in infants versus children and adults with t(4;11) ALL. Oncogene 2001; 20, 2900-2907

  • Von Berg A, Gargallo P, De Prijck B, Vranckx H, Marschalek R, Larippa I, Kluin Ph, Schuuring E, Hagemeijer A. Cryptic t(4;11) encoding MLL-AF4 due to insertion of 5‘ MLL sequences in chromosome 4. A molecular cytogenetic study. Leukemia2001; 15, 595-600

  • Reichel M, Gillert E, Breitenlohner I, Repp R, Greil J, Beck JD, Fey GH, Marschalek R. MRD-Meeting report: „Rapid isolation of chromosomal breakpoints from patient with t(4;11) acute lymphoblastic leukemia: implications for basic and clinical research". Leukemia 2001; 15, 286-288.

 

>

Staff:

  • Prof. Dr. Theo Dingermann
    - Dr. rer. nat. Ilse Zündorf
    - Apotheker Christian Klein
    - Apothekerin Ute Steine

  • HD Dr. Thomas Winckler
    - Dipl. Biol. Monika Baik
    - Dipl. Ing. Oliver Siol
    - Apotheker Ingo Jennes
    - Apothekerin Thanh Chung
    - Julia Bosse (TA)

  • Prof. Dr. Rolf Marschalek
    - Dipl. Biol. Adelheid Bursen
    - Dipl. Biol. Anne Gaußmann
    - Sabine Jakob (TA)
    - Apotheker Sven Moritz
    - Dipl. Ing. Claus Meyer
    - Dipl. Biol. Sebastian Scharf
    - Dipl. Biol. Björn Schneider
    - Dipl. Chem. Jürgen Zech
    - Jens Rabenstein (TA)

>

ZAFES-Coordinator:

 

 

 

INSTITUTES AND
MEDICAL CENTERS

 
 

RHEUMATOLOGY

 
 

GASTROENTEROLOGY - HEPATOLOGY

 
 

PAEDIATRIC HAEMATOL., ONCOL. & HAEMOSTASEOLOGY

 
 

NEURORADIOLOGY

 
 

CLINICAL PHARMACOLOGY

 
 

GENERAL PHARMACOLOGY &
TOXICOLOGY

 
 

IMMUNE PHARMACOLOGY

 
 

INTERDISCIPLINARY PHARMACOLOGY

 
 

PHARMACEUTICAL TECHNOLOGY

 
 

PHARMACEUTICAL CHEMISTRY

 

PHARMACEUTICAL BIOLOGY

 

PROF. DINGERMANN /
PROF. MARSCHALEK

 
 
 

GEORG SPEYER HAUS

 
 

ORGANIC CHEMISTRY &
CHEMICAL BIOLOGY

 
 

NEUROLOGY

 
 

ANESTHESIOLOGY, INTENSIVE CARE AND
PAIN CONTROL

 
 

OBSTETRICS AND GYNECOLOGY

 
 

DEPARTMENT OF DERMATOLOGY & VENEROLOGY

 
 

CARDIOVASCULAR PHYSIOLOGY

 
 

PEDIATRIC PNEUMOLOGY, ALLERGY AND CYSTIC FIBROSIS

MEDICAL VIROLOGY

ECOLOGY, EVOLUTION AND DIVERSITY, DEPARTMENT AQUATIC ECOTOXICOLOGY

 
 

ORAL, CRANIO-
MAXILLOFACIAL AND
FACIAL PLASTIC SURGERY

 
 

GENERAL AND VASCULAR SURGERY

 
 

CLINICAL NEUROANATOMY

 
 

GENERAL PEDIATRICS

 
 

HAEMATOLOGY, ONCOLOGY

 
 

DIAGNOSTIC AND INTERVENTIONAL RADIOLOGY

 
 

BIOCHEMISTRY

 
 

GENERAL PRACTICE

 
 

MPI FOR
BRAIN RESEARCH