Medical Clinic I:
Gastroenterology / Nutritional- and Prevention Medicine
Prof. Dr. med. Dr. oec. troph. Jürgen Stein

Expertise

Development of new immunomodulatory strategies in inflammatory bowel disease
Secondary prevention of colorectal cancer
Anticarcinogenic effects of secondary plant extracts (prebiotics, polyphenols)

Resources and Abilities

2D-Gel electrophoresis
semi-quantitative PCR (Pico-Green), DNA-sequencing, cloning, transfection/transformation
Immunohistochemistry
HPLC
Radioisotope analyser
Infra red absorption spectroscopy
Exhaled hydrogen monitoring
- H2-breath tests, C14-breath tests
FACS
Patchclamp-Studies, Calcium-Imaging (single cells and organotypic slices)
Experimental animal models:
- colitis-models (TNBS, DSS, IL-2)
- cystic fibrosis-model (CFTR-knockout mice)

Publications

Bott C, Rudolph MW, Schirrmacher S, Skalsky B, Petereit H-U, Langguth P, Dressman JB, Stein J. In vivo evaluation of a novel pH- and time-based multi-unit drug deilivery system. Aliment Pharmacol Ther (submitted).
Schröder O, Blumenstein I, Schulte-Bockholt A, Stein J. Combining infliximab and methotrexate in fistulising Crohn's disease resistant or intolerant to azathioprine. Aliment Pharmacol Ther 2004 Feb; 19(3): 295-301.
Schröder O, Stein J. Low dose methotrexate in inflammatory bowel disease: current status and future directions. Am J Gastroenterol 2003 Mar; 98(3): 530-7.
Jordan A, Stein J. Effect of an omega-3 fatty acid containing lipid emulsion alone and in combination with 5-fluorouracil (5-FU) on growth of the colon cancer cell line Caco-2. Eur J Nutr. 2003 Dec; 42(6): 324-31.
Wolter F, Turchanowa L, Stein J. Resveratrol-induced modification of polyamine metabolism is accompanied by induction of c-Fos. Carcinogenesis. 2003 Mar; 24(3): 469-74.
Trojan J, Povse N, Schröder O, Stein J. A new immunological test strip device for the rapid, qualitative detection of faecal occult blood. Z Gastroenterol 2002 Nov; 40(11): 921-4.
Gaschott T, Steinmeyer A, Steinhilber D, Stein J. ZK 156718, a low calcemic, antiproliferative, and prodifferentiating vitamin D analog. Biochem Biophys Res Commun. 2002 Jan 11; 290(1): 504-9.
Wächtershauser A, Akoglu B, Stein J. HMG-CoA reductase inhibitor mevastatin enhances the growth inhibitory effect of butyrate in the colorectal carcinoma cell line Caco-2. Carcinogenesis. 2001 Jul; 22(7): 1061-7.
Gaschott T, Wächtershauser A, Steinhilber D, Stein J. 1,25-Dihydroxycholecalciferol enhances butyrate-induced p21(Waf1/Cip1) expression. Biochem Biophys Res Commun. 2001 Apr 27; 283(1): 80-5.