Institute for Pharmaceutical Chemistry -
Prof. Dr. Michael Karas

>

Expertise

• Identification and characterization of proteins expressed in cells or cell compartments
  
• Mass spectrometry (MALDI and ESI) of intact proteins, peptides, oligosaccharides, drugs and metabolites
  
• Method development for investigation of membrane proteins and membrane protein complexes
  
• In-vitro assay for transcription/translation for search for new inhibitors of the bacterial protein synthesis (antibiotics)
  
• Method development in drug analysis
  
• Liquid chromatography
  
• Gel electrophoresis
  
  

>

Resources and Abilities

• 1D/2D-Gelelectrophoresis
  
• Blue-native Gel electrophoresis
  
• MALDI TOF Mass spectrometry
  
• MALDI TOF-TOF Mass spectrometry
  
• LC-MALDI
  
• Nano-ESI-MS
  
• HPLC-ESI-MS/MS
  
• µ-bore, capillary- and nano-HPLC
  
• TAP Tandem affinity purification for analysis of functional multi-protein complexes
  
  

>

Staff:

• Dr. Ute Bahr (Protein MS, Oligosaccharid MS, Oligonukleotid-MS))
  
• Apothekerin Sabrina Baltruschat (Proteomics)
  
• Dipl. Ing. Tobias Beckhaus (Proteomics)
  
• Apothekerin Sandra Bornemann (Proteomics)
  
• Dipl. Chemiker Thorsten Jaskolla (Grundlagen zum MALDI-Prozess)
  
• Apotheker Björn Meyer (Proteomics, Membranproteine)
  
• Dipl. Biologe Malte Schürken (Proteomics, Membranproteine)
  
  

>

Publications

• Weidlich M, Klammt C, Bernhard F, Karas M, Stein T: Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches. Lett Appl Microbiol 2008; 46: 155-159.
  
• Meyer B, Wittig I, Karas M, Schägger H: Identification of two proteins associated with mammalian ATP synthase. Mol Cell Proteomics 2007; 6: 1690-1699.
  
• Petri J, Kaunzinger A, Niemöller A, Karas M: Quality control of tablets by Near Infrared (NIR)-Spectroscopy. Pharmazie 2005; 60: 743-746.
  
• Hofmann S, Glückmann M, Kausche S, Schmidt A, Corvey C, Lichtenfels R, Huber C, Albrecht C, Karas M, Herr W: Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by nanoLC MALDI MS/MS mass spectrometry. Mol Cell Proteomics 2005; 4: 1888-1897.
  
• Fandino AS, Rais I, Vollmer M, Elgass H, Schägger H, Karas M: LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis. J Mass Spectrom 2005; 40: 1223-1231.
  
• Corvey C, Stein T, Kötter P, Entian KD, Karas M: Carbon source dependent assembly of the Snf1p kinase complex in Candida albicans. J Biol Chem 2005; 280: 25323-25330.
  
• Fandino AS, Rais I, Vollmer M, Elgass H, Schägger H, Karas M: LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis. J Mass Spectrom 2005; 40: 1223-1231.
  
• Hofmann S, Glückmann M, Kausche S, Schmidt A, Corvey C, Lichtenfels R, Huber C, Albrecht C, Karas M, Herr W: Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by nanoLC MALDI MS/MS mass spectrometry. Mol Cell Proteomics 2005; 4: 1888-1897.
  
• Aivaliotis M, C. Corvey, I. Tsirogianni, M. Karas, G: Tsiotis Membrane proteome analysis of the green-sulfur bacterium Chloropium tepidum Electrophoresis 25 2004; 3468-3474.
  
• Dörrschuck A, Schmidt A, Schnürer E, Glückmann M, Albrecht C, Wölfel C, Lennerz V, Lifke A, Di Natale C, Ranieri E, Huber C, Karas M, Herr W: CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA-class Ia/Ib-associated renal carcinoma antigens with ubiquitous or restricted tissue expression. Blood 2004; 104: 2591-2599.
  
• Rais I, Karas M, Schägger H: Two-dimensional gel elctrophoresis for the isolation of integral membrane proteins and mass spectrometric identification Proteomics 2004; 4: 2567-2571.
  
• Keller JH, Karas M, Müller WE, Volmer DA, Eckert GP, Abdel-Tawab M, Blume HH, Dingermann T, Schubert-Zsilavecz M: Determination of Hyperforin in Mouse Brain by Rapid High Performance Liquid chromatography/Tandem Mass Spectrometry. Anal Chem 2003; 75: 6084-6088.
  
• Ekroos K, Ejsing CS, Bahr U, Karas M, Simons K, Shevchenko A: Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation. J Lipid Res 2003; 44: 2181-2192.
  
• Corvey C, Stein T, Düsterhus S, Karas M, Entian KD: Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr. Biochem Biophys Res Commun 2003; 304: 48-54.
  
• Krüger R, Karas M: Ion Formation in MALDI: The Cluster Ionization Mechanism. Chem Rev 2003; 103: 427-439.
  
• Schmidt A, Dülks T, Karas M: Effect of different solution flow rate on analyte ion signals in nano-ESI MS, or : When does ESI turn to Nano-ESI? J Am Soc Mass Spectrom 2003; 14: 492-500.
  
• Pfenninger A, Karas M, Finke B, Stahl B: Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 2 : Application to Isomeric Mixtures.). J Am Soc Mass Spectrom 2002; 13: 1341-1348.
  
• Pfenninger A, Karas M, Finke B, Stahl B: Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 1 : Methodology). J Am Soc Mass Spectrom 2002; 13: 1331-1340.
  
• Schmidt A, Karas M: The influence of Electrostatic Interactions on the Detection of Heme-Globin Complexes in ESI-MS J. Am. Soc. Mass Spectrom 2001; 12: 1092-1098.
  
• Schmidt A, Bahr U, Karas M: The Influence of Pressure in the First Pumping Stage on Analyte Desolvation and Fragmentation in Nano-ESI MS. Anal Chem 2001; 73: 6040-6046.
  
• Abdel Tawab M, Bahr U, Danieli B, Gebhardt S, Karas M, Riva S, Schubert-Zsilavecz M: Electrospray Mass Spectrometry with Consecutive Fragmentation Steps (ES-MSn) as a Tool for Rapid and Sensitive Analysis of Ginsenosides and their Galactosyl Derivatives. Helv Chim Acta 2000; 83: 739-747.
  
• Finke B, Stahl B, Pfenninger A, Karas M, Daniel H, Sawatzki G: Analysis of High-Molecular-Weight Oligosaccharides from Human Milk by Liquid Chromatography and MALDI-MS. Anal Chem 1999; 71: 3755-3762.
  
• Bahr U, Karas M: Differentiation of "Isobaric" Peptides and Human Milk Oligosaccharides by Exact Mass Measurements Using ESI-orthogonal TOF Analysis. Rapid Commun Mass Spectrom 1999; 13: 1052-1058.
  
• Hillenkamp F, Karas M, Beavis RC, Chait BT: Matrix- Assisted Laser Desorption/Ionization Mass Spectrometry of Biopolymers. Anal Chem 1991; 63: 1193A -1203A.