Institute of Biochemistry I: Pathobiochemistry -
Prof. Dr. Bernhard Brüne

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Expertise

• Inflammation associated with malfunctions in oxygen metabolism
  
• Tumor biology linked to macrophage polarization and lipid metabolism
  
• Hypoxia/HIF-1α or HIF-2α (hypoxia inducible factor) and its role in biomedicine
  
• Molecular mechanisms in sepsis development and progression
  
  

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Resources and Abilities

Resources:

• Flow cytometer to analyze surface marker expression, to detect apoptotic cell parameters and quantitate cytokines by Cytometric Bead Array
  
• Laser scanning microscope
  
• Fluorescence microscope with Apotome Upgrade
  
• Hypoxia-chamber

Knockout mice:

• Caspase-1 knockout mouse
  
• Gfi knockout mouse
  
• Conditional HIF-1α knockout mouse
  
• Conditional HIF-2α knockout mouse
  
• Lck-Cre mouse
  
• LysM-Cre mouse
  
• Nrf2 knockout mouse
  
• Conditional PPARγ knockout mouse
  

Animal models:

• Endotoxin-sepsis-model
  
• PyMT-model (mouse breast cancer)
  
  

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Staff

• Dr. Nathalie Dehne (Hypoxia)
  
• Dr. Emeka Igwe (Hypoxia and T cell biology)
  
• Dr. Dmitry Namgaladze (Arteriosclerosis)
  
• Dr. Tobias Schmid (Translation control)
  
• PD Dr. Andreas von Knethen (Sepsis)
  
• Dr. Andreas Weigert (Alternative polarization of macrophages and lipid metabolism)
  
  

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Publications

• Johann AM, Weigert A, Eberhardt W, Kuhn AM, Barra V, von Knethen A, Pfeilschifter JM, Brüne B: Apoptotic cell-derived sphingosine-1-phosphate promotes HuR-dependent cyclooxygenase-2 mRNA stabilization and protein expression. J Immunol 2008; 180: 1239-1248.
  
• Werno C, Zhou J, Brüne B: A23187, ionomycin and thapsigargin upregulate mRNA of HIF-1alpha via endoplasmic reticulum stress rather than a rise in intracellular calcium. J Cell Physiol 2008; 215: 708-714.
  
• Zhou J, Eleni C, Spyrou G, Brüne B: The mitochondrial thioredoxin system regulates nitric oxide-induced HIF-1alpha protein. Free Radic Biol Med 2008; 44: 91-98.
  
• Brüne B, Zhou J: Hypoxia-inducible factor-1alpha under the control of nitric oxide. Methods Enzymol 2007; 435: 463-478.
  
• Herr B, Zhou J, Dröse S, Brüne B: The interaction of superoxide with nitric oxide destabilizes hypoxia-inducible factor-1alpha. Cell Mol Life Sci 2007; 64: 3295-3305.
  
• Namgaladze D, Kollas A, Brüne B: Oxidized LDL attenuates apoptosis in monocytic cells by activating ERK signaling. J Lipid Res 2008; 49: 58-65.
  
• Weigert A, Tzieply N, von Knethen A, Johann AM, Schmidt H, Geisslinger G, Brüne B: Tumor cell apoptosis polarizes macrophages role of sphingosine-1-phosphate. Mol Biol Cell 2007; 18: 3810-3819.
  
• Johann AM, Barra V, Kuhn AM, Weigert A, von Knethen A, Brüne B: Apoptotic cells induce arginase II in macrophages, thereby attenuating NO production. FASEB J 2007; 21: 2704-2712.
  
• Brüne B, Zhou J: Nitric oxide and superoxide: interference with hypoxic signaling. Cardiovasc Res 2007; 75: 275-2782.
  
• von Knethen A, Soller M, Tzieply N, Weigert A, Johann AM, Jennewein C, Köhl R, Brüne B: PPARgamma1 attenuates cytosol to membrane translocation of PKCalpha to desensitize monocytes/macrophages. J Cell Biol 2007; 176: 681-694.
  
• Soller M, Dröse S, Brandt U, Brüne B, von Knethen A: Mechanism of thiazolidinedione-dependent cell death in Jurkat T cells. Mol Pharmacol 2007; 71: 1535-1544.
  
• von Knethen A, Soller M, Brüne B: Peroxisome proliferator-activated receptor gamma (PPAR gamma) and sepsis. Arch Immunol Ther Exp (Warsz) 2007; 55: 19-25.
  
• Preiss S, Namgaladze D, Brüne B: Critical role for classical PKC in activating Akt by phospholipase A2-modified LDL in monocytic cells. Cardiovasc Res 2007; 73: 833-840.
  
• Zhou J, Damdimopoulos AE, Spyrou G, Brüne B: Thioredoxin 1 and thioredoxin 2 have opposed regulatory functions on hypoxia-inducible factor-1alpha. J Biol Chem 2007; 282: 7482-7490.
  
• Namgaladze D, Brüne B: Phospholipase A2-modified low-density lipoprotein activates the phosphatidylinositol 3-kinase-Akt pathway and increases cell survival in monocytic cells. Arterioscler Thromb Vasc Biol 2006; 26: 2510-2516.
  
• Ulrich S, Loitsch SM, Rau O, von Knethen A, Brüne B, Schubert-Zsilavecz M, Stein JM: Peroxisome proliferator-activated receptor gamma as a molecular target of resveratrol-induced modulation of polyamine metabolism. Cancer Res 2006; 66: 7348-7354.
  
• Köhl R, Preiss S, von Knethen A, Brüne B: Oxidized low-density lipoprotein depletes PKCalpha and attenuates reactive oxygen species formation in monocytes/macrophages. Cardiovasc Res 2006; 71: 574-585.
  
• Zhou J, Brüne B: Cytokines and hormones in the regulation of hypoxia inducible factor-1alpha (HIF-1alpha). Cardiovasc Hematol Agents Med Chem 2006; 4: 189-197.
  
• Maccarrone M, Brüne B: Redox regulation by ischemia/reperfusion. Cell Death Differ 2006; 13: 2002-2003.
  
• Weigert A, Johann AM, von Knethen A, Schmidt H, Geisslinger G, Brüne B: Apoptotic cells promote macrophage survival by releasing the antiapoptotic mediator sphingosine-1-phosphate. Blood 2006; 108: 1635-1642.
  
• Köhl R, Zhou J, Brüne B: Reactive oxygen species attenuate nitric-oxide-mediated hypoxia-inducible factor-1alpha stabilization. Free Radic Biol Med 2006; 40: 1430-1442.
  
• Schnitzer SE, Schmid T, Zhou J, Brüne B: Hypoxia and HIF-1alpha protect A549 cells from drug-induced apoptosis. Cell Death Differ 2006; 13: 1611-1613.
  
• Zhou J, Köhl R, Herr B, Frank R, Brüne B: Calpain mediates a von Hippel-Lindau protein-independent destruction of hypoxia-inducible factor-1alpha. Mol Biol Cell 2006; 17: 1549-1558.
  
• Soller M, Tautenhahn A, Brüne B, Zacharowski K, John S, Link H, von Knethen A: Peroxisome proliferator-activated receptor gamma contributes to T lymphocyte apoptosis during sepsis. J Leukoc Biol 2006; 79: 235-243.
  
• Johann AM, von Knethen A, Lindemann D, Brüne B: Recognition of apoptotic cells by macrophages activates the peroxisome proliferator-activated receptor-gamma and attenuates the oxidative burst. Cell Death Differ 2006; 13: 1533-1540.