Institute for Organic Chemistry and Chemical Biology -
Prof. Dr. Joachim Engels

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Expertise

• inhibition of HIV using Hammerhead Ribozymes with 2’modified or fluorated universal nucleoside analogues
  
• hybridization experiments and detection of point mutations
  
• 6-oxocytidinphosphoramidit a CpG oligo desoxynucleotide building block and its potential in asthma therapy
  
  

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Resources and Abilities

• DNA- / RNA-synthesis
  
• HPLC- analytic
  
• MALDI-mass spectrometry
  
• ESI-mass spectrometry
  
  

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Staff

• Dipl.-Chem. Kerstin Jahn-Hofmann (Hybridization experiments and the detection of point mutations)
  
• Dipl.-Chem. Astrid Klöpffer (2´-modified or fluorated universal nucleoside analogues, Hammerhead-Ribozyme)
  
• Gerda Wittel (6-Oxocytidinphosphoramidite as a CpG oligo desoxynucleotide building block)
  
  

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Publications

• Klöpffer AE, Engels JW: Synthesis of 2´-Aminoalkyl Substituted Fluorinated Nucleobases and Their Influence on the Kinetic Properties of Hammerhead Ribozymes. ChemBioChem 2004; in press.
  
• Jahn-Hofmann K, Engels JW: Efficient solid phase synthesis, of cleavable oligodeoxynucleotides based on a novel strategy for the synthesis of 5´-S-(4,4´-Dimethoxytrityl)-mercapto-amidites. Helv.Chim.Acta 2004; in press.
  
• Jahn-Hofmann K, Engels JW: A new concept for DNA-arrays. Nucleosides, Nucleotides & Nucleic Acids. 2003; 5-8, 1479-1482.
  
• Ellinger T, Ermanntraut E, Ehricht R, Engels JW: Jahn-Hofmann K., PCT Int. Appl. 2003;, 165 pp. WO 0318838K.
  
• Uhlmann E, Vollmer J: Recent advances in the development of immunostimulatory oligonucleotides. Curr Opin Drug Discov Devel 2003; 6:204-217.
  
• Jahn-Hofmann K, Engels JW: A new concept for DNA-arrays. Collection Symposium Series 2002; 5 (Chemistry of Nucleic Acid Components): 335-337.
  
• Klebba C, Ottmann OG, Engels JW et al.: Retrovirally Expressed Anti-HIV Ribozymes Confer a Selective Survival Advantage on CD4+ T Cells in vitro. Gene Therapy 2000; 7: 408-416.
  
• Parsch U, Engels JW: pH-Independent Triple-Helix Formation with 6-Oxocytidine as Cytidine Analogue. Chem Eur J 2000; 6:2409-2424.
  
• Mag M, Lücking S, Engels JW: Synthesis and cleavage of an oligodeoxynucleotide containing a bridged internucleotide 5-phosphorthioate linkage. Nucleic Acids Res (1991) 19:1437-1441.