Institute for Organic Chemistry
&
Chemical Biology Prof. Dr. Joachim
Engels
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The research focus of Prof.
Engels group is the modification
of desoxynucleic and ribonucleic
acids. These modifications can
take place at the bases and
the sugars, as well as the backbone
of the DNA. These modified nucleotides
and/or ribonucleotides are incorporated
in DNA or RNA strands (Hammerhead-Ribozym,
RNAi, Antisense, universal bases,
CpG-oligo desoxynucleotide-motifes).
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Expertise
- inhibition of HIV using
Hammerhead Ribozymes with
2’modified or fluorated
universal nucleoside analogues
- hybridization experiments
and detection of point mutations
- 6-oxocytidinphosphoramidit
a CpG oligo desoxynucleotide
building block and its potential
in asthma therapy
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Resources
and Abilities
- DNA- / RNA-synthesis
- HPLC- analytic
- MALDI-mass spectrometry
- ESI-mass spectrometry
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Publications
- Klöpffer, A. E.; Engels,
J.W.; ChemBioChem 2004 in
press; Synthesis of 2´-Aminoalkyl
Substituted Fluorinated Nucleobases
and Their Influence on the
Kinetic Properties of Hammerhead
Ribozymes
- K. Jahn-Hofmann, J. W. Engels;
Helv.Chim.Acta 2004 in press,
Efficient solid phase synthesis;
of cleavable oligodeoxynucleotides
based on a novel strategy
for the synthesis of 5´-S-(4,4´-Dimethoxytrityl)-mercapto-amidites,
- K. Jahn-Hofmann, J. W. Engels;
Nucleosides, Nucleotides &
Nucleic Acids 2003, 5-8, 1479-1482;
A new concept for DNA-arrays.
- T. Ellinger, E. Ermanntraut,
R. Ehricht, J. W. Engels,
K. Jahn-Hofmann; PCT Int.
Appl. 2003, 165 pp. WO 0318838K.
- Uhlmann, E., Vollmer, J.;
Current Opinion in Drug &
Development 2003, 6, 2, 204-217;
Recent advances in the development
of immunostimulatory oligonucleotides
- Jahn-Hofmann, J. W. Engels;
Collection Symposium Series
2002, 5 (Chemistry of Nucleic
Acid Components), 335-337;
A new concept for DNA-arrays.
- Klebba,C.; Ottmann, OG.;
Engels, J.W. et al; Gene Therapy
7, 2000, 408-416; Retrovirally
Expressed Anti-HIV Ribozymes
Confer a Selective Survival
Advantage on CD4+ T Cells
in vitro
- Parsch, U.; Engels, J.W.;
Chem. Eur. J. 2000; 6, 13;
2409-2424pH-Independent Triple-Helix
Formation with 6-Oxocytidine
as Cytidine Analogue
- M. Mag, S. Lücking,
J. W. Engels; Nucleic Acids
Res. 1991, 19 (7), 1437-1441;
Synthesis and cleavage of
an oligodeoxynucleotide containing
a bridged internucleotide
5-phosphorthioate linkage
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Staff:
- Dipl.-Chem. Kerstin Jahn-Hofmann
(Hybridization experiments
and the detection of point
mutations)
- Dipl.-Chem. Astrid Klöpffer
(2´-modified or fluorated
universal nucleoside analogues,
Hammerhead-Ribozyme)
- Gerda Wittel (6-Oxocytidinphosphoramidite
as a CpG oligo desoxynucleotide
building block)
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