Department of Pharmacology, University Mainz
Prof. Dr. med. Leszek Wojnowski

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Expertise

• Pharmacogenetics
  - CYP3A family of drug metabolising enzymes
  - Genetic predictors of anthracycline-induced cardiotoxicity
• Mouse genetics
• Pharmacotherapy
  
  

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Resources and Abilities

• Genotyping using common DNA techniques and SNP-Chips
• RNA-Expression (TaqMan, chips)
• Induction and promoter activity measurements using cells transfected with luciferase constructs
• Drug monitoring by HPLC
• Experimental mouse models. Currently:
  - knockouts of all NOS genes
  - knockout of the gp91 subunit of the NADPH oxidase
  - Braf protooncogene knockout
• histology and immunohistochemistry
• common cloning, transfection, and cell culture techniques
  
  

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Publications

• Kulle B, Schirmer M, Toliat MR, Suk A, Becker Ch, Tzvetkov MV, Brockmöller J, Bickeböller H, Hasenfuss G, Nürnberg P, Wojnowski L: Application of genome-wide SNP arrays for detection of simulated susceptibility loci. Human Mutation, in press
  
  
• Tzvetkov MV, Becker Ch, Kulle B, Nürnberg, Brockmöller J, Wojnowski L: Genome-wide single-nucleotide polymorphism arrays demonstrate high fidelity of multiple displacement-based whole-genome amplification. Electrophoresis 26: 710–715, 2005
  
  
• Kamata T, Kang J, Lee TH, Wojnowski L, Pritchard CA, Leavitt AD. A critical function for B-Raf at multiple stages of myelopoiesis. Blood. [Epub ahead of print, March 22, 2005]
  
  
• Wojnowski L, Turner PC, Pedersen B, Hustert E, Brockmoller J, Mendy M, Whittle HC, Kirk G, Wild PC: Increased levels of aflatoxin-albumin adducts are associated with CYP3A5 polymorphisms in The Gambia, West Africa. Pharmacogenetics 14: 691-700, 2004
  
  
• Wojnowski L, Brockmoller J: Single nucleotide polymorphism characterization by mRNA expression imbalance assessment. Pharmacogenetics 14:267-9, 2004
  
  
• Kamdem LK, Meineke I, Koch I, Zanger UM, Brockmöller, Wojnowski L: Limited contribution of CYP3A5 to the hepatic 6beta-hydroxylation of testosterone. Naunyn Schmiedebergs Arch Pharmacol 370:71-7, 2004
  
  
• Burk O, Koch I, Raucy J, Hustert E, Eichelbaum M, Brockmöller J, Zanger UM, Wojnowski L: The induction of CYP3A5 in human liver and intestine is mediated by the xenobiotic sensors PXR and CAR. J Biol Chem,279:38379-85, 2004
  
  
• Koch I, Weil R, Wolbold R, Brockmöller J, Hustert E, Burk O, Nuessler A, Neuhaus P, Eichelbaum M, Zanger UM, Wojnowski L: Interindividual Variability and Tissue-Specificity in the Expression of Cytochrome P450 3A mRNA. Drug Metab Dispos 30:1108-1114, 2002
  
  
• Burk O, Tegude H, Koch I, Hustert E, Wolbold R, Glaeser H, Klein K, Fromm MF, Nuessler AK, Neuhaus P, Zanger UM, Eichelbaum M, Wojnowski L: Molecular mechanisms of polymorphic CYP3A7 expression in adult human liver and intestine. J Biol Chem 277:24280-8, 2002
  
  
• Wojnowski L, Hustert E, Klein K, Goldammer M, Haberl M, Kirchheiner J, Koch I, Klattig J, Zanger UM, Brockmöller J: Re: Modification of Clinical Presentation of Prostate Tumors by a Novel Genetic Variant in CYP3A4. J Natl Cancer Inst 94:630-631, 2002
  
  
• Hustert E, Haberl M, Burk O, Wolbold R, He YQ, Klein K, Neussler A, Neuhaus P, Klattig J, Eiselt R, Koch I, Zibat A, Brockmöller J, Halpert JR, Zanger UM, Wojnowski L: The genetic determinants of the CYP3A5 polymorphism. Pharmacogenetics 11:773-779, 2001
  
  
• Hustert E, Zibat A, Presecan-Siedel E, Eiselt R, Mueller R, Fuß C, Brehm I, Brinkmann U, Eichelbaum M, Wojnowski L, Burk O: Natural protein variants of pregnane x receptor with altered transactivation activity toward CYP3A4. Drug Metab Dispos 29:1454-9, 2001
  
  
• Eiselt R, Domanski TL, Zibat A, Mueller R, Presecan-Siedel E, Hustert E, Zanger UM, Brockmöller J, Klenk H-P, Meyer UA, Khan KK, He YA, Halpert JR, Wojnowski L: Identification and functional characterization of eight CYP3A4 protein variants. Pharmacogenetics 11:447-58, 2001
  
  
• Gellner K, Eiselt R, Hustert E, Arnold H, Koch I, Haberl M, Deglmann CJ, Burk O, Buntefuss D, Escher S, Bishop C, Koebe H-G, Brinkmann U, Klenk H-P, Kleine K, Meyer UA, Wojnowski L: Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene. Pharmacogenetics 11:111-21, 2001
  
  

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Staff:

• Dr. rer. nat. Anke Kruger
• Dr. rer. nat. Thomas Lang
  

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ZAFES-Coordinator:

• Prof. Dr. med. Leszek Wojnowski
  E-Mail: wojnowski@uni-mainz.de
  

THE RHEIN-MAIN CENTER FOR CLINICAL STUDIES

INNOVATION CENTER FOR BIOTECHNOLOGY FRANKFURT (FIZ)

CENTRAL LABORATORY FOR GERMAN PHARMACISTS